CULTIVATION OF MAMMALIAN CELLS IN DEFINED MEDIA WITH PROTEIN AND NONPROTEIN SUPPLEMENTS

Cultivation of Mammalian Cells in Defined Media with Protein and Nonprotein Supplements

An improved chemically defined basal medium (CMRL-1415) has been used to advantage in studying the effects on trypsinized, newly explanted mouse embryo cells of certain glycoproteins from plasma and serum, certain nonprotein macromolecules, and various combinations of these, in stationary cultures. When protein and nonprotein fractions were separated from fetal calf serum, the entire growth act...

Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media.

Chemically defined media for cultivation of Naegleria gruberi.

Cultivation of amebae of the axenic strain of Naegleria gruberi, NEG-M, was achieved in media consisting entirely of chemically defined components. A complete medium that contains 31 components allows growth with yields up to 5 x 10(6) amebae per ml. A minimal medium gives lower yields but defines 22 components that are essential for continuous cultivation: 11 amino acids, 6 vitamins, hematin, ...

The cultivation of brucellae on chemically defined media.

The selection of a chemically defined medium for nutritional or physiological studies depends upon the objectives of the investigator. The four chemically defined media for the cultivation of brucellae that have been reported during the past decade have tended toward one or the other of two objectives. Either multiple amino acid nitrogen source media have been devised and used to establish the ...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...